Novel Molecular Beacon Probe-Based Real-Time RT-PCR Assay for Diagnosis of Crimean-Congo Hemorrhagic Fever Encountered in India

Table 1

Sequence of primers and MB probe sequence used in developing MB rRT-PCR assay for the diagnosis of CCHF.

Primer/probe	Oligonucleotide sequence (5′-3′)	Nucleotide position (JX051650)𝛽
MB Probe	6-FAM-CGCGATCATCTCATCTTTGTTGTTCACCTCGATCGCG-BHQ-1	71–93
Forward primer	AGTGTTCTCTTGAGTGCTA	32–50
Reverse primer	CCACAAGTCCATTTCCTT	120–137

6-FAM: 6-carboxyfluorescein.
BHQ-1: Black Hole Quencher-1.
Underlined bases are molecular beacon probe stem.
𝛽Nucleotide position with reference to NCBI accession number JX051650.