Research Article
Novel Molecular Beacon Probe-Based Real-Time RT-PCR Assay for Diagnosis of Crimean-Congo Hemorrhagic Fever Encountered in India
Table 2
Intra- and interassay variability for the MB rRT-PCR assay on the basis of Cp values. The limit of detection was determined to be 7.6 copies. The assay was able to generate reproducible results with CV of <5% for both intra- and interassay. The intra-assay variability for TaqMan rRT-PCR assay is also shown on the basis of Cp value. For TaqMan assay the limit of detection is 10-fold lower than MB assay.
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