Novel Molecular Beacon Probe-Based Real-Time RT-PCR Assay for Diagnosis of Crimean-Congo Hemorrhagic Fever Encountered in India

<table class="table-group" id="tab3"><tr><td><table class="table"><tr><td class="thead-hr" colspan="4"><hr/></td></tr><tr class="thead"><td align="left">Assay </td><td align="center">Sensitivity </td><td align="center">Error </td><td align="center">Efficiency</td></tr><tr><td class="thead-hr" colspan="4"><hr/></td></tr><tr><td align="left">MB rRT-PCR assay</td><td align="center">7.6 copies</td><td align="center">0.0869</td><td align="center">1.975</td></tr><tr><td align="left">TaqMan rRT-PCR assay</td><td align="center">76 copies </td><td align="center">0.162</td><td align="center">1.816</td></tr><tr class="table-tr"><td colspan="4"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

Comparison of MB rRT-PCR assay and TaqMan rRT-PCR assay. MB rRT-PCR assay was found to have comparatively higher efficiency, lower error, and 10-fold higher sensitivity than TaqMan rRT-PCR assay.

BioMed Research International

tab3

Table 3

Table 3: Novel Molecular Beacon Probe-Based Real-Time RT-PCR Assay for Diagnosis of Crimean-Congo Hemorrhagic Fever Encountered in India 