Novel Molecular Beacon Probe-Based Real-Time RT-PCR Assay for Diagnosis of Crimean-Congo Hemorrhagic Fever Encountered in India

Table 4

Testing of clinical samples for CCHFV genome using RT-PCR (nested PCR) and TaqMan rRT-PCR for validation of presently developed MB rRT-PCR assay.

Location (districts)	Species	Type of sample	Nested RT-PCR	TaqMan rRT-PCR	MB rRT-PCR	Results
Ahmedabad; Mehsana; Kutch; Dahod; Silvassa	Cattle; Buffalo; Sheep; Goat	Serum	03	03	03	Positive
		Blood	03	03	03
		Ticks	03	03	03
		Serum	178	178	178	Negative
		Blood	12	12	12
		Ticks	13	13	13
Total			212	212	212