Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2014, Article ID 497508, 7 pages
http://dx.doi.org/10.1155/2014/497508
Research Article

Effects of Quercetin on CYP450 and Cytokines in Aroclor 1254 Injured Endometrial Cells of the Pregnant Rats

1College of Veterinary Medicine, Agricultural University of Hebei, Baoding, Hebei 071001, China
2Institute of Traditional Chinese Veterinary Medicine, Agricultural University of Hebei, Baoding, Hebei 071001, China

Received 22 September 2013; Revised 16 December 2013; Accepted 24 December 2013; Published 10 March 2014

Academic Editor: Hartmut Jaeschke

Copyright © 2014 Lina Xu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Polychlorinated biphenyls (PCBs) are widespread persistent residual environmental pollutants, which affect seriously the growth and reproductive alterations in humans and animals. Aroclor 1254 is a commercial mixture of PCBs. Quercetin is a flavonoid, which acts on estrogen receptors and causes the development of estrogen-related diseases. In this paper, the primary cultured endometrial cells in the pregnant rats were isolated and Aroclor 1254 was used to induce the injured endometrial cells model. The cells were treated with gradient quercetin, the viability of the endometrial cells, the expressions of CYP450, the contents of TNF-α, IL-6, estradiol (E2), and progesterone (P4) were measured. It showed that the viability of the cultured endometrial cells, the expression of CYP1A1 and CYP2B1, and the contents of TNF-α, E2, and IL-6 in the injured endometrial cells increased with the treatment of quercetin. It shows that quercetin has protective effect on the injured endometrial cells in the pregnant rats, this provide a basis on herbal medicine protection for animal reproductive diseases caused by environmental endocrine disruptors.