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BioMed Research International
Volume 2014, Article ID 503162, 14 pages
http://dx.doi.org/10.1155/2014/503162
Research Article

MicroRNA Profiling Reveals Unique miRNA Signatures in IGF-1 Treated Embryonic Striatal Stem Cell Fate Decisions in Striatal Neurogenesis In Vitro

1Department of Neuroscience, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
2Department of Pediatrics-Neurology, Baylor College of Medicine, Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, 1250 Moursund Street, Room 1250, Houston, TX 77030, USA
3Department of Pathology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
4Laboratory of Neuropsychopharmacology, FFCLRP, University of Sao Paulo (USP), Avenida Bandeirantes 3900, Monte Alegre, Ribeirao Preto, SP 14040-900, Brazil
5Department of Medical Microbiology, School of Medical Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
6Departments of Pediatrics-Neurology and Neuroscience, Program in Developmental Biology, Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, 1250 Moursund Street, Room 1250, Houston, TX 77030, USA
7Center for Neuroscience Services and Research, Universiti Sains Malaysia, Sultanah Zainab 2 Road, 16150 Kubang Kerian, Kelantan, Malaysia
8Neuroscience Department, Universiti Sains Malaysia Hospital, USM Hospital Road, 16150 Kubang Kerian, Kelantan, Malaysia

Received 10 April 2014; Revised 25 June 2014; Accepted 2 July 2014; Published 1 September 2014

Academic Editor: Aijun Wang

Copyright © 2014 Soumya Pati et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The striatum is considered to be the central processing unit of the basal ganglia in locomotor activity and cognitive function of the brain. IGF-1 could act as a control switch for the long-term proliferation and survival of EGF + bFGF-responsive cultured embryonic striatal stem cell (ESSC), while LIF imposes a negative impact on cell proliferation. The IGF-1-treated ESSCs also showed elevated hTERT expression with demonstration of self-renewal and trilineage commitment (astrocytes, oligodendrocytes, and neurons). In order to decipher the underlying regulatory microRNA (miRNA)s in IGF-1/LIF-treated ESSC-derived neurogenesis, we performed in-depth miRNA profiling at 12 days in vitro and analyzed the candidates using the Partek Genome Suite software. The annotated miRNA fingerprints delineated the differential expressions of miR-143, miR-433, and miR-503 specific to IGF-1 treatment. Similarly, the LIF-treated ESSCs demonstrated specific expression of miR-326, miR-181, and miR-22, as they were nonsignificant in IGF-treated ESSCs. To elucidate the possible downstream pathways, we performed in silico mapping of the said miRNAs into ingenuity pathway analysis. Our findings revealed the important mRNA targets of the miRNAs and suggested specific interactomes. The above studies introduced a new genre of miRNAs for ESSC-based neuroregenerative therapeutic applications.