Single Amino Acid Arginine Deprivation Triggers Prosurvival Autophagic Response in Ovarian Carcinoma SKOV3

Figure 1

Effect of arginine deprivation on SKOV3 cell proliferation. The growth of SKOV3 cells in arginine-sufficient complete medium (CM), in arginine-free medium (AFM), and the ability of SKOV3 cells to rescue cell proliferation after different periods of arginine starvation was assessed by counting only the viable cells. After the indicated periods of incubation in AFM, cells were shifted to a fresh CM and allowed to proliferate for additional 72 h (AFM→CM (72 h)). Viable cells were determined by the trypan blue exclusion test. The initial number of cells (time-point zero) was considered as 100%. Data from three independent experiments in triplicate.