Single Amino Acid Arginine Deprivation Triggers Prosurvival Autophagic Response in Ovarian Carcinoma SKOV3
Figure 2
Expression of the key genes of arginine anabolism in SKOV3 cells. (a) Specific mRNA levels determined by RT-PCR analysis as described in Section 2. Cells were cultured in arginine-free medium (AFM) for 24 h or in arginine-sufficient complete medium (CM). Human hepatocarcinoma HepG2 cells (which express urea cycle enzymes) were used as a positive control. GAPDH expression was used as an internal loading control. (b) Scheme of arginine biosynthesis in the urea cycle. ARG1: arginase I, ARG2: arginase II, OTC: ornithine transcarbamylase, ASS: argininosuccinate synthetase, and ASL: argininosuccinate lyase.
