Figure 3: Staining patterns of CD133, VEGF-A, CD34, and microvessel density in chronic nasopharyngitis (inflammation) and NPC tissues. (a) The expression of CD133 by immunofluorescence staining (green). Nuclei were counterstained by DAPI (blue). HE staining of parallel sections. Immunoperoxidase staining of (b) VEGF-A (brown) and (c) CD34 (brown). Arrows show CD34-positive vessels. The red dotted line is border between cancer nest and stromal area. Original magnification is 200x (VEGF-A in upper panels, CD34) and VEGF-A enlarged in the lower panels (400x). Scale bar represents 50 μm. Ca: cancer cells. Graphs represent average and SD of microvessels/field and CD34 area fraction (%) for inflammation ( ) and NPC tissues ( ). values were calculated by the Mann-Whitney test in comparison to chronic nasopharyngitis tissues. ; .