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BioMed Research International
Volume 2014, Article ID 514190, 6 pages
Research Article

Attachment of Asaia bogorensis Originating in Fruit-Flavored Water to Packaging Materials

Institute of Fermentation Technology and Microbiology, Lodz University of Technology, 171/173 Wolczanska Street, 90 924 Lodz, Poland

Received 2 June 2014; Revised 27 August 2014; Accepted 29 August 2014; Published 11 September 2014

Academic Editor: Petr Kralik

Copyright © 2014 Dorota Kregiel et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The objective of this study was to investigate the adhesion of isolated spoilage bacteria to packaging materials used in the food industry. Microorganisms were isolated from commercial fruit-flavored mineral water in plastic bottles with flocks as a visual defect. The Gram-negative rods were identified using the molecular method through the amplification of a partial region of the 16S rRNA gene. Based on the sequence identity (99.6%) between the spoilage organism and a reference strain deposited in GenBank, the spoilage isolate was identified as Asaia bgorensis. Experiments on bacterial adhesion were conducted using plates made of glass and polystyrene (packaging materials commonly used in the beverage industry). Cell adhesion ability was determined using luminometry, plate count, and the microscopic method. The strain of A. bogorensis was characterized by strong adhesion properties which were dependent on the surface type, with the highest cell adhesion detected on polystyrene.