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BioMed Research International
Volume 2014 (2014), Article ID 519098, 9 pages
Research Article

In Vitro Antifungal Evaluation of Seven Different Disinfectants on Acrylic Resins

1Department of Prosthodontics, Faculty of Dentistry, Gazi University, Emek, 06510 Ankara, Turkey
2Department of Dentomaxillofacial Radiology, Faculty of Dentistry, Gazi University, 06510 Ankara, Turkey
3Department of Microbiology, Faculty of Dentistry, Gazi University, 06510 Ankara, Turkey

Received 28 February 2014; Revised 19 May 2014; Accepted 22 May 2014; Published 5 June 2014

Academic Editor: Tong Zhang

Copyright © 2014 A. Z. Yildirim-Bicer et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objective. The aim of this study was to evaluate alternative methods for the disinfection of denture-based materials. Material and Methods. Two different denture-based materials were included in the study. Before microbial test, the surface roughness of the acrylic resins was evaluated. Then, the specimens were divided into 8 experimental groups , according to microorganism considered and disinfection methods used. The specimens were contaminated in vitro by standardized suspensions of Candida albicans ATCC#90028 and Candida albicans oral isolate. The following test agents were tested: sodium hypochlorite (NaOCl 1%), microwave (MW) energy, ultraviolet (UV) light, mouthwash containing propolis (MCP), Corega Tabs, 50% and 100% white vinegar. After the disinfection procedure, the number of remaining microbial cells was evaluated in CFU/mL. Kruskal-Wallis, ANOVA, and Dunn’s test were used for multiple comparisons. Mann Whitney U test was used to compare the surface roughness. Results. Statistically significant difference was found between autopolymerised and heat-cured acrylic resins. The autopolymerised acrylic resin surfaces were rougher than surfaces of heat-cured acrylic resin. The most effective disinfection method was 100% white vinegar for tested microorganisms and both acrylic resins. Conclusion. This study showed that white vinegar 100% was the most effective method for tested microorganisms. This agent is cost-effective and easy to access and thus may be appropriate for household use.