Figure 6: Western blotting for assessing protein expressions of inflammatory biomarkers including MMP-9 (a), (b), NF-κB (c), (d), and TNF-α (e), (f) in normal remote myocardium, infarct and peri-infarct areas of both groups. Note the significantly lower expressions of MMP-9 and TNF-α in normal than in controls and tac-treated animals (* versus other groups, all ), and significantly higher expressions of these two biomarkers in controls than in tac-treated animals († versus ‡, all ) (a), (b), (e), (f). Note the significantly lower expressions of NF-κB in normal than in the infarct areas in both groups and the peri-infarct area in controls (* versus †. all ). NF-κB expression is significantly lower in the peri-infarct areas in tac-treated animals (* versus †. ) but there is no significant differences between two groups in the infarct areas (c), (d).