Development of a Reverse Transcription Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Subtype H7N9 Avian Influenza Virus
Figure 1
Detection of H7N9 influenza viruses by H7-RT-LAMP. Viral RNAs were extracted from the H7N9 influenza viruses and were amplified using H7-RT-LAMP. LAMP products were detected by a real-time turbidity assay using an LA-320c (a) and a fluorescence assay (b). CH1: A/CK/Shanghai/S1053/2013 (H7N9); CH2: A/EN/Shanghai/S1088/2013 (H7N9); CH3: A/PG/Shanghai/S1069/2013 (H7N9); CH4: A/DK/Anhui/SC702/2013 (H7N9); CH5: A/CK/Jiangsu/SC002/2013 (H7N9); CH6: A/Anhui/1/2013 (H7N9); CH7: A/African starling/England/983/79 (H7N1); CH8, negative control.
