Development of a Reverse Transcription Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Subtype H7N9 Avian Influenza Virus
Figure 4
Specificity of the N9-RT-LAMP assay for different subtype influenza viruses and other avian respiratory viruses. Viral RNAs were extracted from the representative influenza viruses of avian origin (subtypes N1–9) (a) and other avian respiratory viruses (b). The RT-LAMP reaction was assessed based on the fluorescence intensity. N1, A/Goose/Guangdong/1/96 (H5N1); N2, A/Turkey/Wisconsin/1/66 (H9N2); N3, A/Duck/Germany/1215/73 (H2N3); N4, A/Turkey/Ontario/6118/68 (H8N4); N5, A/Mallard/Gurjev/263/82 (H14N5); N6, A/Duck/Czech/56 (H4N6); N7, A/equine/jingfang/1/74 (H7N7); N8, A/Duck/Ukraine/1/63 (H3N8); N9, A/Duck/Memphis/546/76 (H11N9); N9 (H7N9), A/chicken/Shanghai/S1053/2013;.
