Research Article

Development of a Reverse Transcription Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Subtype H7N9 Avian Influenza Virus

Table 2

Details of RT-LAMP and RT-PCR primers designed for detection of NA gene sequences of N9 subtype influenza viruses.

Primer nameaGenome positionLength(s) Sequences (5′ to 3′)

N9-F318-merGTTTCATGCGACCCAGAT
N9-B320-merCATGGCAACTAGTACTTGAC
N9-FIP41-merTTCCGTTTGAGTGTTTCCCTCGAATGCAGGTTCTATGCTCT
N9-BIP39-merCAGTATCGCGCCCTGATAAGCCACCCAATGCATTCCACC
N9-LB23-merTGGCCACTATCATCACCGCCCAC
N9-LF18-merTGATTGTTGTTCCTTGGC
N9 F22-merATAATGAAACAAACATCACCAA
N9 R22-merAGCATAGAACCTGCATTCATCT

The primers of N9-F3, N9-B3, N9-FIP, N9-BIP, N9-LB, and N9-LF were for RT-LAMP Primers. N9-F and N9-R were applied to RT-PCR.