The Cytotoxicity of Benzaldehyde Nitrogen Mustard-2-Pyridine Carboxylic Acid Hydrazone Being Involved in Topoisomerase IIα Inhibition
Figure 3
BNMPH redox activity and induction of DNA fragmentation. (a) ROS generation by Fenton reaction; the ROS product was measured by DCF fluorescence. (b) The ROS induced DNA fragmentation in the presence or absence of BNMPH; the fragmented DNA was separated by agarose gel electrophoresis and visualized by EB staining, and the concentration is as indicated. (c) DNA fragmentation in vivo was evaluated by comet assay, top: control, and bottom: in the presence of 50 μM BNMPH.
