Mesenchymal Stem Cell-Induced Doxorubicin Resistance in Triple Negative Breast Cancer
Figure 1
Adipose-derived mesenchymal stem cells-secreted conditioned medium induced doxorubicin resistance in MDA-MB-231 cells. Cells were treated by 200 nM doxorubicin with L15 (a) or MSCM medium (b) in a time-dependent manner and cell viability was examined by performing crystal violet staining. (c) Cells were treated by 200 nM doxorubicin with MSC-ad conditioned medium for 24 hours and cell viability was also examined by performing crystal violet staining. Graphs showed mean ± SEM of three independent experiments. to doxorubicin-untreated group; to doxorubicin in MSCM group. CM, conditioned medium from adipose-derived mesenchymal stem cells (MSC-ad); MSCM, mesenchymal stem cell medium (fresh MSC culture medium).
