Insulin Preconditioning Elevates p-Akt and Cardiac Contractility after Reperfusion in the Isolated Ischemic Rat Heart

Figure 1

Experimental protocol. The Pre-Ins group received 0.5 U/L insulin in KH buffer prior to 15 min no-flow ischemia for 20 min followed by 20 min of reperfusion. The Post-Ins group received 0.5 U/L insulin during the reperfusion period only. The control group was perfused with KH buffer throughout.