Plasma Albumin Induces Cytosolic Calcium Oscilations and DNA Synthesis in Human Cultured Astrocytes
Figure 3
Cellular mechanisms involved in cytosolic calcium change. (a) Response to BPA (20 mg/mL) in 0-calcium (0 + 10−3 mol/L EGTA). The first pulse of BPA can induce an increase in , but not the second, performed with presumably intracellular empty calcium-stores. (b) Response to BPA in presence of 50 × 10−6 mol/L ryanodine. (c) Absence of response to BPA (20 mg/mL) in presence of 10−5 mol/L 2-APB. (d) BPA fails to induce increases in presence of heparin (1 mg/mL). (e) Response to BPA in presence of LY-364947 (30 × 10−6 mol/L) showed a slight reduction in the maximum height of calcium increase (inset); for paired Student’s -test ( cells obtained from 3 patients). (f) Diagram showing the effects of several agents acting onto receptor/second-messenger systems ( cells, obtained from 8 patients); for Student’s -test. Empty bars show application time of BPA (20 mg/mL) and solid bars show application of different agents (in min).
