Plasma Albumin Induces Cytosolic Calcium Oscilations and DNA Synthesis in Human Cultured Astrocytes
Figure 4
Functionality of astrocytes after long-duration incubation in heparin. (a) Perfusion of glutamate (5 × 10−4 mol/L, grey bars) and BPA (20 mg/mL, empty bars) in control induced increase. Then, we perfused heparin (1 mg/mL) during 24 hours. Eight minutes after heparin, glutamate was able to induce a response similar to the control one, but albumin failed to modify the . At 12 hours and at 23 hours and 45 minutes in heparin, we obtained the same result. Nevertheless, at 24 hours and 10 minutes, that is, 10 minutes after having washed out heparin, both agents induced a increase similar to the control. Time of heparin indicated at the top. Grey bars show application of glutamate and empty bars show application of BPA. Solid bars show presence of heparin. (b) Diagram showing the maximum height of increase induced by glutamate (solid dots) and BPA (empty dots).
