Apoptosis Induction by Polygonum minus Is Related to Antioxidant Capacity, Alterations in Expression of Apoptotic-Related Genes, and S-Phase Cell Cycle Arrest in HepG2 Cell Line
Figure 4
Histogram of annexin-V and FITC/PI flow cytometry of HepG2 cells exposed to F7. HepG2 cells () were incubated with F7 of P. minus (25 μg/mL, equivalent to ) or (a) control for (b) 24, (c) 48 and (d) 72 h at 37°C. Lower left quadrant in each panel represents viable cells which excluded PI and were negative for annexin V-FITC binding. Upper right quadrant contains nonviable, necrotic cells or late stage apoptotic cells, positive for annexin V-FITC/PI uptake. Lower right quadrant contains early apoptotic cells, annexin V-FITC positive and PI negative. One experiment is representative of three independent experiments.
