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BioMed Research International
Volume 2014 (2014), Article ID 539697, 9 pages
http://dx.doi.org/10.1155/2014/539697
Research Article

Virulence Factors Associated with Pediatric Shigellosis in Brazilian Amazon

1Instituto Leônidas e Maria Deane—Fiocruz Amazônia, Rua Terezina 476, Adrianópolis, 69.057-070 Manaus, AM, Brazil
2Programa de Pós Graduação em Imunologia Básica e Aplicada (PPGBA-UFAM), Avenida General Rodrigo Octávio 6200, Coroado I, 69.077-000 Manaus, AM, Brazil
3Departamento de Ciências Básicas da Saúde, Universidade Federal de Juiz de Fora, Câmpus Governador Valadares, Rua Israel Pinheiro 2000, Bairro Universitário, 35010177 Governador Valadares, MG, Brazil

Received 28 November 2013; Accepted 9 April 2014; Published 29 April 2014

Academic Editor: Angel Cataldi

Copyright © 2014 Carolinie Batista Nobre da Cruz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Shigellosis is a global human health problem and the incidence is highest among children. In the present work, main Shigella virulence genes was examined by PCR and compared to symptoms of pediatric shigellosis. Thirty Shigella isolates were identified from an etiologic study at which 1,339 children ranging 0–10 years old were enrolled. S. flexneri was the most frequent species reaching 60.0% of isolates, 22.2% were S. sonnei, and 6.6% were both S. dysenteriae and S. boydii. All Shigella infected children had diarrhea, but not all were accompanied by others symptoms of bacillary dysentery. Among major virulence genes, the PCR typing revealed ipaBCD was present in all isolates, followed by IpaH7.8, set-1A, set-1B, sen/ospD3, virF, and invE. The pathogenic potential of the ShET-1B subunit was observed in relation to dehydration () and ShET-2 related to the intestinal injury () evidenced by the presence of bloody diarrhea. Our results show associations among symptoms of shigellosis and virulence genes of clinical isolates of Shigella spp.