BioMed Research International / 2014 / Article / Tab 1

Research Article

Genetic Testing in Hereditary Breast and Ovarian Cancer Using Massive Parallel Sequencing

Table 1

Summary of sequencing runs and coverage results of the validation set.

Run 1Run 2Run 3
BRCAMASTRv1.2BRCAMASTRv2.0BRCAMASTRv2.0

Samples788
Amplicons1699494
Passed filter reads11800678777100771
Mapped reads11337478698100344

Coverage (number of reads/amplicon)
Minimum191317
Mean96,98102,7132,27
Maximum277445414
Standard deviation coverage35,846,9270,51
Amplicons <38 reads (%)32 (2.7)32 (4.2)13 (1.72)