Activity of Tea Tree (Melaleuca alternifolia) Essential Oil against L3 Larvae of Anisakis simplex

Table 2

In vitro effect of Melaleuca alternifolia essential oil and terpinen-4-ol on L3 of Anisakis simplex survival. Results are expressed as average mortality ± standard deviation.

Compound	Concentration (μL/mL)	Average mortality (%); (𝑛=90 L3)
		24 h	48 h
Control		3.3±10	5.6±6.7
Melaleuca alternifolia EO	10	100^∗	100^∗
	7	93±5.8^∗	100^∗
	5	52.3±38.4^∗	81.1±24.2^∗
	4	30±20.9	38.3±11.7^∗
	3	18.3±20.4	21.7±17.2
	2	10±15.5	5±8.4
	1	7.7±10.9	10±13.2
	0.5	2.2±6.8	2.2±6.8
Terpinen-4-ol	10	0	0
	1	0	0
	0.1	0	0

∗Indicates values that differ from the control (𝑃<0.05).
Levamisole was used as positive control reference drug at the dose of 100 μg/mL obtaining 100% of mortality after 24 h.
n: total number of L3 used to test every concentration.