Induction of Boosted Immune Response in Mice by Leptospiral Surface Proteins Expressed in Fusion with DnaK
Figure 3
Analysis of IgG production induced in mice by recombinant proteins. The sera from mice immunized with fused-proteins were analyzed by ELISA (a) and Western blotting (b). In (a) wells were coated with DnaK or surface proteins, as depicted; in (b) blotted proteins are DnaK (lane 1), Lsa21 (lane 2), rLIC10494 (lane 3), Lp95 C-terminus region (lane 4), and rLIC12730 (lane 5). M: molecular mass protein marker. In both methods, proteins were probed with the respective antifusion protein serum (1 : 20,000 dilution) and the reactions were developed with HRP-conjugated anti-goat IgG (1 : 10,000) (ELISA) and HRP-conjugated anti-mouse IgG (1 : 5,000) (Western).
