Induction of Boosted Immune Response in Mice by Leptospiral Surface Proteins Expressed in Fusion with DnaK

Figure 6

Analysis of cytokine profiles from spleen cells supernatant. Spleen cells from immunized mice were isolated and cultured in 24-well tissue culture plates. After 48 h, cell-free culture supernatants were collected, and the level of cytokines, IFN-γ and IL-10, was measured by ELISA. For statistical analysis, concentration values for the recombinant fusion proteins-immunized group stimulated with the same protein were compared with those immunized and stimulated with the corresponding protein alone by the two-tailed 𝑡-test.