Induction of Boosted Immune Response in Mice by Leptospiral Surface Proteins Expressed in Fusion with DnaK

Table 1

Gene locus, protein given name, NCBI reference sequence number, features, sequence of the primers employed for DNA amplification, and molecular mass of expressed recombinant proteins.

Gene locus^1	Recombinant protein given name	NCBI reference sequence number^2	Description/function	Sequence of primers for PCR amplification and restriction cloning sites	Recombinant protein molecular mass
LIC10524	DnaK	YP_000508	Molecular chaperone DnaK	F: 5′GGATCCGCTATGGAAGGTGGAGACCCGGTC3′ (BamHI); R: 5′CCATGG    GATATC  TCACAGCTG  CGGACCCGGACCCTTTTTCTCATCGTCTAC3′; (NcoI, EcoRV and PvuII)	68.9 kDa
LIC10368	Lsa21^a	YP_000355	Putative lipoprotein	F: 5′GGATCC    CAGCTG  GCTATGG  CCTGTCCGGATGAAAAAAAAG3′; (BamHI and PvuII); R: 5′CCATGG    GATATCTCAAAATACATTCACACGAATATCTC3′; (NcoI and EcoRV)	19.8 kDa
LIC10494	rLIC10494^b	YP_000478	Putative lipoprotein	F: 5′GGATCC    CAGCTG  GCTATGG  TAATCTTTGGCAACAAAAC3′; (BamHI and PvuII); R: 5′CCATGG    GATATC  TCA  TTTGTTTTCAGATTTTTTC3′; (NcoI and EcoRV)	25.1 kDa
LIC12690	Lp95 c-terminal^c	YP_002611	Putative lipoprotein with a domain of unknown function (DUF1554)	F: 5′GGATCC    CAGCTG  GCTATGG  AAAATGGAGAAGACGTTAC3′; (BamHI and PvuII); R: 5′CCATGG    GATATC  TCA  TTGTTCCACACAAAGAATG3′; (NcoI and EcoRV)	43.6 kDa
LIC12730	rLIC12730^d	YP_002650	Hypothetical protein with TPR motif	F: 5′GGATCC    CAGCTG  GCTATGG  GACTTCCCAATTTCTCTATTC3′  (BamHI and PvuII); R: 5′CCATGG    GATATC  TCA  TTTCGTAAAAAATCCAGCTTC3′  (NcoI and EcoRV)	75.7 kDa

1http://aeg.lbi.ic.unicamp.br/world/lic/ [25].
2http://www.ncbi.nlm.nih.gov/protein/. Protein BLAST-http://www.ncbi.nlm.nih.gov/blast/Blast.cgi [26, 27].
aAtzingen et al., 2008 [18], Atzingen et al., 2012 [28].
bAtzingen et al., 2010 [20], Vieira et al., 2010 [29].
cAtzingen et al., 2009 [19].
dAtzingen et al., 2010 [20], Vieira et al., 2010 [29], Felix et al., 2011 [30].