An Historical Perspective on How Advances in Microscopic Imaging Contributed to Understanding the Leishmania Spp. and Trypanosoma cruzi Host-Parasite Relationship
Figure 5
Visualization of the T. cruzi intracellular life cycle using field-emission scanning electron microscopy. (a) Tissue cultured trypomastigotes (TCTs) (blue) egress from Vero cells (light brown). (b) Intracellular amastigotes (red) of T. cruzi hosted by Vero cells (light brown). Infected cells were fixed with 4% paraformaldehyde and then subjected to electron scanning processing. Briefly, samples were dehydrated in an ethanol series, subjected to critical-point drying from CO2 and gold sputtering. In (b), samples processed as in (a) were fractured by adhesive tape and then gold sputtered. Scale bars, 10 μm.
