Variability in Myosteatosis and Insulin Resistance Induced by High-Fat Diet in Mouse Skeletal Muscles
Figure 6
Effect of high-fat diet (HFD) on insulin signal transduction in the soleus and tibialis anterior muscles of control mice and HFD mice. Total IRS-1 protein expression and Ser307 phosphorylation (panel (a)), total Akt protein expression and Ser473 phosphorylation (panel (b)), and total GSK-3β protein expression and Ser9 phosphorylation (panel (c)) were analyzed by Western blot on muscle homogenates. Densitometric analysis of the bands is expressed as relative optical density (O.D.), corrected for the corresponding tubulin contents and normalized using the related control band. The data are means SD of 10 animals. ; .
