C-Terminal Domain Swapping of SSB Changes the Size of the ssDNA Binding Site

Figure 3

Protein purity. Coomassie Blue-stained SDS-PAGE (15%) of the purified KpSSB (lane 1), StSSB (lane 2), PaSSB (lane 3), KpSSBnStSSBc (lane 4), KpSSBnPaSSBc (lane 5), and molecular mass standards (M) are shown. The sizes of the standard proteins, from the top down, are as follows: 55, 40, 35, 25, 15, and 10 kDa. The purified SSBs migrated between the 25 and 15 kDa standards on the SDS-PAGE.