Research Article
TolC Promotes ExPEC Biofilm Formation and Curli Production in Response to Medium Osmolarity
Table 1
Bacterial strains and plasmids.
| Strain or plasmid | Description | Source or reference |
| Strain | | | ExPEC strain PPECC42 | Wild-type (WT), porcine origin, CmS | Lab stock | PPECC42tolC | Mutant with a 158-bp fragment deleted from the whole ORF of the tolC gene in PPECC42, CmS | This study | Cm-tolC | PPECC42tolC strain containing plasmid pHSG-tolC, CmR | This study | χ7213 | Thi-1 thr-1 leuB6 fhuA21 lacY1 glnV44 asdA4 recA1 RP4 2-Tc::Mu[λpir] KmR | [22] | χ7213-tolC | χ7213 containing suicide vector pRE112ΔtolC | This study | DH5α | F−, φ80dlacZΔM15, (lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(, ), phoA, supE44, λ −, thi-1, gyrA96, relA1 | Takara Bio | Plasmid | | | pRE112 | oriT oriV Δasd CmR SacB, suicide vector | [22] | pRE tolC | pRE112-inserted disrupted tolC gene in Kpn I and Sac I sites | This study | pHSG396 | ori lacZ CmR | Takara Bio | pHSG-tolC | CmR | This study |
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