Timing of Peripheral Blood Stem Cell Yield: Comparison of Alternative Methods with the Classic Method for CD34⁺ Cell Determination

Figure 3

Determination of CD34⁺ and CD133⁺ cells. Determination of CD34⁺ and CD133⁺ cells using flow cytometry (modified ISHAGE protocol). Tricolor flow cytometry CD34⁺ (FITC)/CD45⁻ (PC5)/CD133⁺ (PE). ((a), (b)) the population of CD34⁺ cells. ((c), (d)) the population of CD133⁺ cells. ((e), (f)) the population of CD34⁺/CD133⁺ cells. FITC = fluorescein isothiocyanate; PE = phycoerythrin; PC5 = phycoerythrin-cyanine5; FS Lin = forward-scatter linear scale; SS Lin = side-scatter linear scale.

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