Phylogenetic and In Silico Functional Analyses of Thermostable-Direct Hemolysin and tdh-Related Encoding Genes in Vibrio parahaemolyticus and Other Gram-Negative Bacteria
Table 1
Comparison of the deduced amino acid sequences of the products of the tdh genes taken from GenBank.
Particulars of tdh genes
Positions of signal peptide
Positions of mature protein sequence
3
4
23
23
28
34
43
50
89
99
108
112
116
119
136
147
162
163
165
Pandemic strains
gi∣21326604∣1998 (O3:K6)
H
Q
S
T
Q
K
N
E
S
G
N
N
E
G
N
I
K
H
Q
gi∣21326606∣1998 (O3:K6)
H
Q
S
T
Q
K
N
E
S
G
N
N
E
G
N
I
K
H
Q
gi∣21326600∣2002 (O4:K68)
H
Q
S
T
Q
K
N
E
S
G
N
N
E
G
N
I
K
H
Q
gi∣21326602∣2002 (O1:KUT)
H
Q
S
T
Q
K
N
E
S
G
N
N
E
G
N
I
K
H
Q
gi∣380448060∣2011
H
Q
S
T
Q
K
N
E
S
G
N
N
E
G
N
I
K
H
Q
gi∣28901233∣RIMD, TDH S, 1996 (O3:K6)
H
Q
S
T
Q
K
N
E
S
G
N
N
E
G
N
I
K
H
Q
gi∣28901169∣RIMD, TDH A, 1996 (O3:K6)
Y
R
F
T
N
E
N
K
H
D
D
N
E
D
N
I
K
H
Q
Nonpandemic strains
gi∣48478∣1992
H
Q
S
T
Q
K
N
E
S
G
N
N
E
G
N
I
K
H
Q
gi∣155291∣1993
H
Q
S
T
Q
K
N
E
S
G
N
N
E
G
N
I
K
H
Q
gi∣21326608∣1980 (O3:K6)
H
Q
S
T
Q
K
N
E
S
D
N
N
E
G
N
I
K
H
Q
giI∣217197∣1985
H
Q
S
T
Q
K
N
E
S
D
N
N
E
G
N
I
K
H
Q
gi∣30171235∣ATCC 17803, 2003
H
Q
S
T
Q
K
N
E
S
D
N
N
E
G
N
I
K
H
Q
gi∣21326594∣2001 (O4:K8)
H
Q
S
T
Q
K
N
E
S
D
N
N
E
G
N
I
K
H
Q
gi∣375809176∣2011
H
Q
S
T
Q
K
N
E
S
D
N
N
E
G
N
I
K
H
Q
gi∣21326598∣ 2001 (O5:K15)
H
Q
S
T
Q
K
N
E
S
D
N
N
E
G
N
I
K
H
Q
gi∣21326596∣2001 (O4:K13)*
H
Q
S
T
Q
K
N
E
S
D
N
N
E
G
N
V
—
—
—
gi∣155295∣Vm-TDH, 1991
Y
Q
F
T
K
K
D
E
S
D
N
N
E
D
N
V
E
H
R
gi∣39748669∣2003
Y
R
F
T
N
E
N
K
H
D
D
N
E
D
N
I
K
H
Q
gi∣217193∣1990
Y
R
F
T
N
E
N
K
H
D
D
S
E
D
N
I
K
H
Q
gi∣380448056∣2011
Y
R
F
T
N
E
N
K
H
D
D
N
E
D
N
I
K
H
Q
gi∣48483∣1990
Y
R
F
T
N
K
N
K
N
D
N
N
E
D
D
I
K
Y
N
gi∣155240∣VcNAG-TDH, 1991
Y
R
F
T
N
K
N
K
N
D
N
N
E
D
D
I
K
Y
N
gi∣48481∣1990
Y
R
F
A
N
K
N
K
R
D
N
N
K
D
N
I
E
H
K
Truncated tdh sequence; Vm, V. mimicus; VcNAG, V. cholerae non-O1 and non-O139; Bang, Bangladesh; RIMD, RIMD2210633. Known serovars are mentioned in parentheses.
