Epigenetic Silencing of CXCR4 Promotes Loss of Cell Adhesion in Cervical Cancer
Figure 3
Reactivation of CXCR4. (a) Expression of CXCR4 at transcription (upper panel) and protein level (lower panel) in cervical cancer cell lines. (b) Reactivation of CXCR4 by AZA and/or TSA treatments. Unmethylated HeLa does not show significant change of CXCR4 transcription, and methylated SiHa, ME-180, and C-33A showed reactivation of CXCR4. (c) Fluorocytometry analysis showed reactivation of CXCR4 protein in SiHa after AZA and TSA treatment. (d) Fluorocytometry analysis showed reactivation of CXCR4 protein in C-33A after AZA and TSA treatment. Note the significant reactivation of CXCR4 (maximum vertical deviation between two curves) after the drug treatments. Data were analysed by Kolmogorov Samirnov (KS) test statistics. “” represents the maximum vertical deviation between two curves.
