Epigenetic Silencing of CXCR4 Promotes Loss of Cell Adhesion in Cervical Cancer

Figure 5

Cell adhesion assay. (a) Expression of CXCR4 and SDF-1α in cultured normal cervical cells (NC65 and NC66) and HEK293 by semiquantitative reverse transcriptase PCR; β-Actin was used as internal control. (b, c) Adhesion of NC65 and NC66 cells after AMD3100 treatment. (d, e) Adhesion of NC65 and NC66 cells in presence of reciprocal conditioned media. (f, g) Adhesion of untreated and TSA treated C-33A cells in presence of conditioned media of NC65. (h) Comparison of cell adhesion in untreated and TSA treated C-33A cells in presence of conditioned media of NC65. (i, j) Adhesion of untreated and TSA treated C-33A cells in presence of conditioned media of HEK293. (k) Comparison of cell adhesion in untreated and TSA treated C-33A cells in presence of conditioned media of HEK293. ^∗𝑃 value ≤ 0.05; ^∗∗𝑃 value ≤ 0.005.

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