Assessment of Free Radical Scavenging Potential and Oxidative DNA Damage Preventive Activity of Trachyspermum ammi L. (Carom) and Foeniculum vulgare Mill. (Fennel) Seed Extracts

Figure 7

Protective effect of T. ammi (AASE, AMSE, and AAcSE) and F. vulgare (FASE, FMSE, and FAcSE) seed extracts at 1 mg/mL concentrationon oxidative damage to calf thymus DNA. Lane 1 : 0.5 μg native calf thymus DNA + 50 mM phosphate buffer, lane 2: 0.5 μg DNA + 2 mM FeSO₄ + 30% H₂O₂ + 50 mM phosphate buffer + AAcSE (1 mg/mL), lane 3: 0.5 μg DNA + 2 mM FeSO₄ + 30% H₂O₂ + 50 mM phosphate buffer + AMSE (1 mg/mL), lane 4: 0.5 μg DNA + 2 mM FeSO₄ + 30% H₂O₂ + 50 mM phosphate buffer + AASE (1 mg/mL), lane 5: 0.5 μg DNA + 2 mM FeSO₄ + 30% H₂O₂ + 50 mM phosphate buffer + FAcSE (1 mg/mL), lane 6: 0.5 μg DNA + 2 mM FeSO₄ + 30% H₂O₂ + 50 mM phosphate buffer + FMSE (1 mg/mL), lane 7: 0.5 μg DNA + 2 mM FeSO₄ + 30% H₂O₂ + 50 mM phosphate buffer + FASE (1 mg/mL), and lane 8: 0.5 μg DNA + 2 mM FeSO₄ + 30% H₂O₂ + 50 mM phosphate buffer.