Oxidatively Modified Proteins in the Serous Subtype of Ovarian Carcinoma
Figure 2
(a) Western blot analysis of expression of reactive carbonyl groups in the cytosolic fraction of serous ovarian carcinoma tissues. Lane M: protein marker; Lane 1: DNPH-derivatized lysate of normal tissue; Lane 2: underivatized lysate of normal tissue; Lane 3: DNPH-derivatized lysate of cystadenoma tissue: Lane 4: underivatized lysate cystadenoma tissue; Lane 5: DNPH-derivatized lysate of borderline tissue; Lane 6: underivatized lysate of borderline tissue; Lane 7: DNPH-derivatized lysate of malignant tissue; Lane 8: underivatized lysate of malignant tissue. (b) The relative optical density on the level of reactive carbonyl proteins using the UN-SCAN-IT system.
