Depletion of Arginine by Recombinant Arginine Deiminase Induces nNOS-Activated Neurotoxicity in Neuroblastoma Cells
Figure 2
Effect of rADI on nNOS-activated NO production in differentiated SH-SY5Y cells. Different buffers (1 mM arginine, arginine-free, or 1 mM arginine plus 1 mU/mL rADI) were prepared in magnesium-free Krebs-Henseleit buffer with 30 μM glycine 24 h prior to the experiments and incubated at 37°C. The differentiated cells were washed with Mg2+-free Krebs-Henseleit buffer twice followed by exposure to untreated control or 1 mM NMDA in the prepared buffers, respectively, for 1 h. The supernatant was collected for NO measurement using the DAN method. The data are the mean ± SEM ( in each group). versus arginine-containing buffer; arg: arginine; NS: no significant difference.
