Cofactor Independent Phosphoglycerate Mutase of Brugia malayi Induces a Mixed Th1/Th2 Type Immune Response and Inhibits Larval Development in the Host
Figure 3
Stage specific expression of Bm-iPGM. (a) Western blot displaying expression of Bm-iPGM. M: standard protein marker; Mf: microfilariae, L3: infective larvae, and Ad: adult parasites. (b) 1.0% agarose gel displaying Bm-iPGM amplification; Bm-iPGM gene was amplified from cDNA of three major life stages of B. malayi using specific primers. Mf: microfilariae, L3: infective larvae, Ad: adult parasites, and M: standard DNA marker. (c) Bm-iPGM in excretory-secretory products of adult parasite. Western blot was done to confirm presence/absence of iPGM enzyme in the ES product of B. malayi. Anti-Bm-iPGM antibody raised in mouse showed reactivity with the purified recombinant protein as well as the ES product of adult parasite. P1: purified recombinant Bm-iPGM, P2: adult worm ES product.
