Cofactor Independent Phosphoglycerate Mutase of Brugia malayi Induces a Mixed Th1/Th2 Type Immune Response and Inhibits Larval Development in the Host
Figure 4
Seroreactivity of Bm-iPGM with human bancroftian sera pooled from 10 filarial patients of each category. (a) Western blots showing cross-reactivity of recombinant Bm-iPGM with various categories of human bancroftian sera. L1: standard protein marker; L2: nonendemic normal; L3: endemic normal; L4: microfilaraemic asymptomatic; L5: microfilaraemic symptomatic; L6 amicrofilaraemic symptomatic patients. (b) Bm-iPGM specific ELISA showing reactivity of Bm-iPGM with human bancroftian sera taken from human subjects belonging to various categories; MFC: microfilaraemic carrier, AMFCS: amicrofilaraemic symptomatic, EN: endemic normal, and NEN: nonendemic normal individuals. Serum (1 : 200) from ten individuals per clinical category was tested in ELISA for reactivity with recombinant Bm-iPGM. All the three groups from filarial endemic area revealed elevated levels of anti-Bm-iPGM antibodies.
