Cofactor Independent Phosphoglycerate Mutase of Brugia malayi Induces a Mixed Th1/Th2 Type Immune Response and Inhibits Larval Development in the Host
Figure 6
Flow cytometric analysis of ROS generation by peritoneal macrophages and splenic lymphocyte surface staining for T and B cells. (a) Representative FACS histogram for ROS generation from each group is displayed and values closer to mean are represented. (b) Reactive oxygen species produced by the peritoneal macrophages (1 × 106/mL) isolated from for all groups were loaded with probe DCF-DA and ROS generation was evaluated on day 30 when the animals were euthanized. Bm-iPGM immunization led to activation of macrophages which significantly generated reactive oxygen species (). (c) CD4+ T cell marker, (d) CD8+ T cell marker, and (e) CD19+ B cell marker. Significant increase was noticed in the number of CD4+ T cells () and CD8+ T cells () from Bm-iPGM immunized animals. Though a marginal expansion in B cell population was observed, it was statistically significant ().
