Synchronization by Food Access Modifies the Daily Variations in Expression and Activity of Liver GABA Transaminase
Figure 2
Analysis of 24 h profile of GABA-T activity in the liver of AL and DRF rats. GABA-T activity was measured in both the homogenate (a) and mitochondrial fraction (b) of the livers from AL and DRF rats by a spectrophotometric assay over a 24 h period. Rats were subjected to a 12 h : 12 h regime of light : dark. The shaded zone represents the dark phase. Food availability for RFS group is indicated by dark boxes (from 12:00 to 14:00 h). Controls of food condition are shown (F and F + R). Average values are represented as dashed line for AL group and a dotted line for RFS group. Graphs show the mean ± SEM of 4 rats per time point. Significant differences () are indicated as follows: *: DRF versus AL by one-way ANOVA and post hoc Bonferroni test, in both the homogenate and mitochondrial fraction; a: RFS versus AL by two-way ANOVA and post hoc Bonferroni test in the mitochondria fraction only; b: F versus F + R by Student’s t-test.
