Synchronization by Food Access Modifies the Daily Variations in Expression and Activity of Liver GABA Transaminase
Figure 3
Analysis of the 24 h profile of relative mRNA expression of GABA-T in the liver of rats under AL and DRF conditions. Relative mRNA levels were determined by RT-qPCR and normalized to Rps18 expression. The shaded zone represents the dark phase. Food availability for DRF group is indicated by dark boxes (from 12:00 to 14:00 h). Graphs show the mean ± SEM of 6 to 8 rats per time point. Average values are represented as a dashed line for the AL group and a dotted line for DRF group. Significant differences () are indicated as follows: *: DRF versus AL by one-way ANOVA and post hoc Bonferroni test, in both the homogenate and mitochondrial fraction; a: RFS versus AL by two-way ANOVA and post hoc Bonferroni test; #: DRF versus F + R at 14:00, by the Student’s t-test.
