Synchronization by Food Access Modifies the Daily Variations in Expression and Activity of Liver GABA Transaminase

Table 1

Chronobiological analysis of liver GABA-T parameters: mRNA, protein amount, and activity.

	GABA-T mRNA		GABA-T protein				GABA-T activity
			Hg		Mt		Hg		Mt
	AL	DRF	AL	DRF	AL	DRF	AL	DRF	AL	DRF
Rhythm (%)	—	—	—	—	—	—	—	54.8	40.3	38.4
Mesor	0.9	0.8	1.1	1.5	0.7	0.9	5.2	5.4	2.4	2.0
Amplitude	0.3	—	—	—	—	—	—	1.55	0.48	0.50
Acrophase (h:min)	7:40/15:15/22:50	—	—	—	—	—	—	9:56	5:56	4:14
Period	8 h	—	—	—	—	—	—	24 h	24 h	24 h

Chronos-Fit analysis was performed to evaluate rhythmicity for GABA-T parameters in liver. Acrophases in GABA-T mRNA from AL group were repeated each 7 h with 40 min. Hg: liver homogenate, Mt: mitochondrial fraction, AL: ad libitum, and DRF: daytime restricted feeding. (—) means that no rhythmic pattern was detected.