Research Article

Synergistic Antimicrobial Action of Chlorhexidine and Ozone in Endodontic Treatment

Figure 3

In vitro evaluation of antimicrobial activity of ozone (A) and chlorhexidine (B) against E. faecalis by flow cytometry. Distribution of fluorescence intensity from bacterial cells treated with ozone (A) for 24, 60, 120, and 180 seconds ((b), (c), (d), and (e), resp.), stained with DiBAC4 (A1) and PI (A2); and cells treated with 0.2 (f) and 2% (g) of chlorhexidine (B) for 3 minutes, stained with DiBAC4 (B1) and PI (B2). Fluorescence of untreated cells stained with fluorescent marker is represented by (a).
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