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BioMed Research International
Volume 2014, Article ID 595186, 13 pages
Research Article

Isolation and Biochemical Characterization of a New Thrombin-Like Serine Protease from Bothrops pirajai Snake Venom

1Centro de Estudos de Biomoléculas Aplicadas à Saúde, CEBio, Fundação Oswaldo Cruz, Fiocruz Rondônia e Departamento de Medicina, Universidade Federal de Rondônia, UNIR, Rua da Beira 7176, Bairro Lagoa, 76812-245 Porto Velho, RO, Brazil
2Departmento de Biologia Celular e Molecular, Instituto de Biologia, Universidade Federal Fluminense, 24210-130 Niteroi, RJ, Brazil
3Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, 09210-170 Santo André, SP, Brazil
4Escola de Artes, Ciências e Humanidades, USP, 03828-000 São Paulo, SP, Brazil
5Universidade Federal de São João Del Rei, UFSJ, Campus Alto Paraopeba, 36420-000 Ouro Branco, MG, Brazil
6Institute for Research in Biomedicine (IRB Barcelona), 08028 Barcelona, Spain
7CIBER-BBN, Barcelona Science Park, 08028 Barcelona, Spain
8Proteomic Platform, Barcelona Science Park, 08028 Barcelona, Spain
9Department of Organic Chemistry, University of Barcelona, 08028 Barcelona, Spain
10School of Chemistry, University of KwaZulu Natal, Durban 4001, South Africa

Received 9 July 2013; Revised 20 September 2013; Accepted 1 December 2013; Published 26 February 2014

Academic Editor: Edward G. Rowan

Copyright © 2014 Kayena D. Zaqueo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Figure 8 displays the energy profiles calculated employing ProSA web server for the initial and final models. The energy values higher than 0 of the initial model followed by same values lower than 0 for the final model indicate that the MD simulation was essential to stabilize the 3D structure.

  1. Supplementary Figures