Angiotensin II Removes Kidney Resistance Conferred by Ischemic Preconditioning

Figure 3

Plasma creatinine concentrations 24 hours after I/R in non-IPC and IPC mice. IPC or non-IPC mice were exposed to either 30 minutes of bilateral renal ischemia/reperfusion (I/R) or a sham-operation (Sham). At 10 minutes before the second surgery, mice were treated with AngII (3 mg/kg body weight, BW), losartan (25 mg/kg BW), AngII plus losartan (AngII + losartan), or vehicle. (a) 24 hours after second surgery, kidney sections were PAS-stained. Images were taken of the outer medulla of the kidneys. (b) Ten fields per kidney were randomly selected to evaluate tubular damage. (c) To determine plasma creatinine concentrations, plasma was collected at 24 hours after the second surgery. Scale bar: 50 μm. Values are means ± SEM . versus vehicle-treated sham in non-IPC; versus vehicle-treated ischemia in non-IPC or IPC.