Angiotensin II Removes Kidney Resistance Conferred by Ischemic Preconditioning
Figure 4
Levels of oxidative stress after I/R in non-IPC and IPC kidneys. IPC or non-IPC mice were exposed to 30 minutes of bilateral renal ischemia/reperfusion (I/R) or a sham-operation (Sham). Ten minutes before second surgery, mice were treated with AngII (3 mg/kg body weight, BW), losartan (Lo, 25 mg/kg BW), AngII plus losartan (AngII + Lo), or vehicle. 24 hours after second surgery, lipid peroxidation was evaluated by assessing (a) MDA levels and (b) Nox2, Nox4, and nitrotyrosine (NT) expression by western blot. GAPDH expression was used to confirm equal protein loading. ((c)–(e)) The densities of the blots were quantified using the ImageJ program. S indicates sham. Values are means ± SEM . versus sham in non-IPC; versus vehicle-treated ischemia in non-IPC or IPC.
