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BioMed Research International
Volume 2014, Article ID 606575, 8 pages
http://dx.doi.org/10.1155/2014/606575
Research Article

Effect of Cocoa Butter and Sunflower Oil Supplementation on Performance, Immunoglobulin, and Antioxidant Vitamin Status of Rats

1Pharmacology and Toxicology Department, Veterinary Faculty, Kirikkale University, 71451 Kirikkale, Turkey
2Biochemistry Department, Veterinary Faculty, Kirikkale University, 71451 Kirikkale, Turkey
3Animal Nutrition and Nutritional Diseases Department, Veterinary Faculty, Kirikkale University, 71451 Kirikkale, Turkey
4Physiology Department, Veterinary Faculty, Kirikkale University, 71451 Kirikkale, Turkey

Received 28 February 2014; Revised 27 June 2014; Accepted 29 June 2014; Published 16 July 2014

Academic Editor: Cristiano Capurso

Copyright © 2014 Ebru Yıldırım et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study investigated the effects of cocoa butter and sunflower oil alone and in combination on performance, some biochemical parameters, immunoglobulin, and antioxidant vitamin status in Wistar rats. Forty-eight male rats were assigned to four groups, consisting of 12 rats with 3 replicates. Control received balanced rat diet without oil, cocoa butter group received 3.5% cocoa butter, sunflower oil group received 3.5% sunflower oil, the last group received 1.75% sunflower oil + 1.75% cocoa butter supplementation in the rat diet for 8 weeks. The total feed consumption in sunflower oil group was statistically lower than in the other groups. The serum creatinine level was decreased in cocoa butter group compared to control. Triglyceride and VLDL cholesterol levels were decreased in only sunflower oil and only cocoa butter groups as compared to control. The level of Ig M was statistically lower in cocoa butter and cocoa butter + sunflower oil groups than in control and sunflower oil groups. There were no statistically important difference in vitamin concentrations among trial groups. It was concluded that the supplementation of cocoa butter in diet decreased Ig M level, while the supplementation of cocoa butter and sunflower oil alone decreased the triglyceride and VLDL cholesterol levels.