A Fast, Reliable, and Sensitive Method for Detection and Quantification of <i>Listeria monocytogenes</i> and <i>Escherichia coli</i> O157:H7 in Ready-to-Eat Fresh-Cut Products by MPN-qPCR

<table>Standard curve and amplification plot of the 96 bp<i> fliC</i> gene fragment by qPCR amplification of serially diluted purified DNA of<i> Escherichia coli</i> O157:H7 represented as log of genome equivalents/reaction. Trend line equation and the corresponding square regression coefficient (<svg height="15.975" id="M7" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 17.4 15.975" width="17.4" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,15.775)"><path d="M627 18l-10 -26q-79 6 -116 27t-69 76q-41 71 -71 138q-13 29 -27.5 39t-42.5 10h-46l-27 -145q-13 -74 -2.5 -88.5t78.5 -20.5l-6 -28h-271l5 28q66 6 82.5 21.5t30.5 87.5l71 387q12 66 2 78.5t-77 19.5l8 28h233q102 0 147 -29q65 -43 65 -129q0 -69 -45.5 -117
t-115.5 -72q40 -86 66 -133q39 -68 65 -101q28 -37 73 -51zM491 483q0 67 -33.5 101t-91.5 34q-35 0 -51 -10q-13 -8 -20 -48l-45 -245h49q71 0 113 28q79 52 79 140z" id="x1D445"></path></g>
<g transform="matrix(.012,-0,0,-.012,11.013,7.613)"><path d="M412 140l28 -9q0 -2 -35 -131h-373v23q112 112 161 170q59 70 92 127t33 115q0 63 -31 98t-86 35q-75 0 -137 -93l-22 20l57 81q55 59 135 59q69 0 118.5 -46.5t49.5 -122.5q0 -62 -29.5 -114t-102.5 -130l-141 -149h186q42 0 58.5 10.5t38.5 56.5z" id="x32"></path></g>
</svg>) are shown.</table>

BioMed Research International

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Figure 2

Figure 2: A Fast, Reliable, and Sensitive Method for Detection and Quantification of <i>Listeria monocytogenes</i> and <i>Escherichia coli</i> O157:H7 in Ready-to-Eat Fresh-Cut Products by MPN-qPCR 

Figure 2 | A Fast, Reliable, and Sensitive Method for Detection and Quantification of Listeria monocytogenes and Escherichia coli O157:H7 in Ready-to-Eat Fresh-Cut Products by MPN-qPCR 